Coding

Part:BBa_K177032:Design

Designed by: Michael Lower   Group: iGEM09_Warsaw   (2009-08-04)


Internalin A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2247
    Illegal XbaI site found at 234
    Illegal SpeI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2247
    Illegal NheI site found at 423
    Illegal SpeI site found at 48
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2247
    Illegal BglII site found at 231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2247
    Illegal XbaI site found at 234
    Illegal SpeI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2247
    Illegal XbaI site found at 234
    Illegal SpeI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 219


Design Notes

Biobrick restriction sites need to be removed from the CDs by site directed mutagenesis


Source

References